the superior colliculus (Figures 3j and 4).
s in a highlight GFP expressing RPE cells outside the injected area. The square in a defines the area displayed with higher magnification in b. GFP expressing RPE cells in c and d are located within the injected area. (e,f) Neuroretina flatmount and corresponding section displaying fluorescent photoreceptor cells. Vans Shoes
Bar 200 dLGN, dorsal lateral geniculat.
No GFP signal was detected outside the visual pathway. In the lateral geniculate nucleus, the GFP signal did not colocalize with cellular bodies of neurons, astrocytes, or oligodendrocytes, as confirmed by immunofluorescent staining using the specific markers, NeuN, GFAP, and Olig2, respectively (Supplementary Figure S1). This result suggests that the GFP signal was present only in nerve fibers that are the axonal projections of ganglion cells in the retina. The animals were killed at 2 months after the injection, and the brains were removed and cut into 100 coronal slices and examined for GFP expression using laser scanning confocal microscopy. Images shown in a correspond to coronal slices at the indicated location on the brain image. c 1 and c 2 correspond to two locations on the same slice c.
Arrows in f indicate photoreceptor nuclei. (g,h) Neuroretina flatmount and corresponding Vans Black
(i) Neuroretina flatmount displaying fluorescent axons of the ganglion cells forming the optic nerve. (j) Transverse section through the optic nerve showing GFP signal in axons. Bar 100 AAV, adeno associated virus. Only a few cells displayed GFP expression at the needle insertion site. The brains were cut into coronal slices and examined for GFP expression using laser scanning confocal microscopy. A strong GFP signal was observed along the visual pathway in the optic nerve, the optic chiasm, the suprachiasmatic nucleus, the Vans Black High Tops optic tract, the ventral and dorsal lateral geniculate nucleus, and Vans All Black For Men
section showing fluorescent ganglion cells and cells of the inner nuclear layer. Arrows in h indicate ganglion cells and cells of the inner nuclear layer.
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