Klickportalen K3 - Klick Data AB

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f.

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used in the PCR, each sample was also used Vans Era Pro Navy

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Vector DNA was detected in the left lateral geniculate nucleus by PCR analysis of frozen tissue from dog D2, thereby indicating that the vector traveled from the right retina along the visual pathway into the contralateral hemisphere of Vans Old Skool Black Girls

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the brain (Figure 7a). Furthermore, reverse transcription PCR analysis of RNA extracted from both the lateral geniculate nuclei of D2 clearly demonstrated the presence of GFP transcripts in the lateral geniculate nucleus contralateral to the injected eye (Figure Vans Era Pro Red 7b). (a) PCR analysis of frozen tissue from dog D2. PCR was carried out using primers that amplified a 424 bp region within the GFP sequence. As control, for an equal amount of DNA as that Vans Era Black Grey

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e nucleus; OC, optic chiasm; OT, optic tract; SC, superior colliculus; SCN, suprachiasmatic nucleus; vLGN, ventral lateral geniculate nucleus. AAV, adeno associated virus. The brains of the dogs were cut into coronal slices (Supplementary Figure S2) and analyzed for GFP expression using laser scanning confocal microscopy. The GFP signal was detected in nerve fibers in the optic nerve, in the optic chiasm, within the contralateral hemisphere in the optic tract, in the nerve fibers and cellular bodies in the lateral geniculate nucleus, and in the superior colliculus (Figure 5, Table 1, and Supplementary Table S1). No GFP signal was observed in the contralateral visual cortex. This conclusion is supported and confirmed by the detection of a GFP signal in nerve fibers of the optic radiation (Figure 5e).

The location of GFP positive neurons was restricted to the A layer of the left lateral geniculate nucleus, which receives its projections from ganglion cells of the contralateral right retina (data not shown). Inserts show the slab in which the GFP signal was detected. Circles within the inserts indicate the location of the GFP signal. LGN, lateral geniculate nucleus; OC, optic chiasm; ON, optic nerve; OR, optic radiation; OT, optic tract; SC, superior colliculus. Bar 50 AAV, adeno associated virus.

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